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probe cs p1  (MedChemExpress)


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    Structured Review

    MedChemExpress probe cs p1
    <t>Bufalin</t> probe <t>CS-P1</t> retains the antitumor activity of bufalin in neuroblastoma. (A) Chemical structure of bufalin and CS-P1. (B) Comparison of antitumor efficacy of bufalin and CS-P1. (C) In-gel fluorescence (azide-fluor 488); targeted proteins were fluorescently labeled. When co-incubated with bufalin and CS-P1, fluorescence was decreased. In-gel staining with Coomassie blue was used as a control. Data are presented as the mean ± SD (n=3). IC 50 , half maximal-inhibitory concentration.
    Probe Cs P1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/probe cs p1/product/MedChemExpress
    Average 90 stars, based on 1 article reviews
    probe cs p1 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Bufalin exerts antitumor effects in neuroblastoma via the induction of reactive oxygen species-mediated apoptosis by targeting the electron transport chain"

    Article Title: Bufalin exerts antitumor effects in neuroblastoma via the induction of reactive oxygen species-mediated apoptosis by targeting the electron transport chain

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4745

    Bufalin probe CS-P1 retains the antitumor activity of bufalin in neuroblastoma. (A) Chemical structure of bufalin and CS-P1. (B) Comparison of antitumor efficacy of bufalin and CS-P1. (C) In-gel fluorescence (azide-fluor 488); targeted proteins were fluorescently labeled. When co-incubated with bufalin and CS-P1, fluorescence was decreased. In-gel staining with Coomassie blue was used as a control. Data are presented as the mean ± SD (n=3). IC 50 , half maximal-inhibitory concentration.
    Figure Legend Snippet: Bufalin probe CS-P1 retains the antitumor activity of bufalin in neuroblastoma. (A) Chemical structure of bufalin and CS-P1. (B) Comparison of antitumor efficacy of bufalin and CS-P1. (C) In-gel fluorescence (azide-fluor 488); targeted proteins were fluorescently labeled. When co-incubated with bufalin and CS-P1, fluorescence was decreased. In-gel staining with Coomassie blue was used as a control. Data are presented as the mean ± SD (n=3). IC 50 , half maximal-inhibitory concentration.

    Techniques Used: Activity Assay, Fluorescence, Labeling, Incubation, Staining, Concentration Assay

    Chemical proteomics highlights the ETC as a potential target. (A) Workflow diagram of nanoLC-MS/MS. (B) Venn diagram of proteins identified in three experimental repeats. (C) Gene Ontology analysis annotated cellular components enriched by CS-P1. (D) Diagram of ETC and the possible targets of CS-P1. (E) Seahorse assay of oxygen consumption and (F) OCR decrease after administration of bufalin in SK-N-BE cells. Rotenone was used as a positive control. (G) Intracellular ROS detection and (H) increase in ROS levels after CS-P1 treatment (329 nM, 72 h; magnification, x200). (I) Mitochondrial membrane potential detection with JC-1 probes and (J) reduction after CS-P1 treatment (329 nM, 72 h; magnification, x200). Data are presented as the mean ± SD (n=3). *** P<0.001 vs. DMSO. ATP5J, ATP synthase-coupling factor 6; COX7C, cytochrome c oxidase subunit 7C; DMSO, dimethyl sulfoxide; ETC, electron transport chain; LC-MS/MS, liquid chromatography-tandem mass spectrometry; OCR, oxygen consumption rate; ROS, reactive oxygen species; SDHD, succinate dehydrogrenase complex subunit D; TBTA, Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine; TCEP, Tris(2-carboxyethyl) phosphine.
    Figure Legend Snippet: Chemical proteomics highlights the ETC as a potential target. (A) Workflow diagram of nanoLC-MS/MS. (B) Venn diagram of proteins identified in three experimental repeats. (C) Gene Ontology analysis annotated cellular components enriched by CS-P1. (D) Diagram of ETC and the possible targets of CS-P1. (E) Seahorse assay of oxygen consumption and (F) OCR decrease after administration of bufalin in SK-N-BE cells. Rotenone was used as a positive control. (G) Intracellular ROS detection and (H) increase in ROS levels after CS-P1 treatment (329 nM, 72 h; magnification, x200). (I) Mitochondrial membrane potential detection with JC-1 probes and (J) reduction after CS-P1 treatment (329 nM, 72 h; magnification, x200). Data are presented as the mean ± SD (n=3). *** P<0.001 vs. DMSO. ATP5J, ATP synthase-coupling factor 6; COX7C, cytochrome c oxidase subunit 7C; DMSO, dimethyl sulfoxide; ETC, electron transport chain; LC-MS/MS, liquid chromatography-tandem mass spectrometry; OCR, oxygen consumption rate; ROS, reactive oxygen species; SDHD, succinate dehydrogrenase complex subunit D; TBTA, Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine; TCEP, Tris(2-carboxyethyl) phosphine.

    Techniques Used: Tandem Mass Spectroscopy, Positive Control, Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry



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    probe cs p1  (MedChemExpress)
    90
    MedChemExpress probe cs p1
    <t>Bufalin</t> probe <t>CS-P1</t> retains the antitumor activity of bufalin in neuroblastoma. (A) Chemical structure of bufalin and CS-P1. (B) Comparison of antitumor efficacy of bufalin and CS-P1. (C) In-gel fluorescence (azide-fluor 488); targeted proteins were fluorescently labeled. When co-incubated with bufalin and CS-P1, fluorescence was decreased. In-gel staining with Coomassie blue was used as a control. Data are presented as the mean ± SD (n=3). IC 50 , half maximal-inhibitory concentration.
    Probe Cs P1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/probe cs p1/product/MedChemExpress
    Average 90 stars, based on 1 article reviews
    probe cs p1 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Bufalin probe CS-P1 retains the antitumor activity of bufalin in neuroblastoma. (A) Chemical structure of bufalin and CS-P1. (B) Comparison of antitumor efficacy of bufalin and CS-P1. (C) In-gel fluorescence (azide-fluor 488); targeted proteins were fluorescently labeled. When co-incubated with bufalin and CS-P1, fluorescence was decreased. In-gel staining with Coomassie blue was used as a control. Data are presented as the mean ± SD (n=3). IC 50 , half maximal-inhibitory concentration.

    Journal: International Journal of Molecular Medicine

    Article Title: Bufalin exerts antitumor effects in neuroblastoma via the induction of reactive oxygen species-mediated apoptosis by targeting the electron transport chain

    doi: 10.3892/ijmm.2020.4745

    Figure Lengend Snippet: Bufalin probe CS-P1 retains the antitumor activity of bufalin in neuroblastoma. (A) Chemical structure of bufalin and CS-P1. (B) Comparison of antitumor efficacy of bufalin and CS-P1. (C) In-gel fluorescence (azide-fluor 488); targeted proteins were fluorescently labeled. When co-incubated with bufalin and CS-P1, fluorescence was decreased. In-gel staining with Coomassie blue was used as a control. Data are presented as the mean ± SD (n=3). IC 50 , half maximal-inhibitory concentration.

    Article Snippet: Bufalin (MedChemExpress), N-acetyl-L-cysteine (NAC; cat. no. S0077; Beyotime Institute of Biotechnology) and the bufalin-derived probe CS-P1 were dissolved in dimethyl sulfoxide (DMSO) at a stock concentration of 100 mM and diluted with medium before experimentation.

    Techniques: Activity Assay, Fluorescence, Labeling, Incubation, Staining, Concentration Assay

    Chemical proteomics highlights the ETC as a potential target. (A) Workflow diagram of nanoLC-MS/MS. (B) Venn diagram of proteins identified in three experimental repeats. (C) Gene Ontology analysis annotated cellular components enriched by CS-P1. (D) Diagram of ETC and the possible targets of CS-P1. (E) Seahorse assay of oxygen consumption and (F) OCR decrease after administration of bufalin in SK-N-BE cells. Rotenone was used as a positive control. (G) Intracellular ROS detection and (H) increase in ROS levels after CS-P1 treatment (329 nM, 72 h; magnification, x200). (I) Mitochondrial membrane potential detection with JC-1 probes and (J) reduction after CS-P1 treatment (329 nM, 72 h; magnification, x200). Data are presented as the mean ± SD (n=3). *** P<0.001 vs. DMSO. ATP5J, ATP synthase-coupling factor 6; COX7C, cytochrome c oxidase subunit 7C; DMSO, dimethyl sulfoxide; ETC, electron transport chain; LC-MS/MS, liquid chromatography-tandem mass spectrometry; OCR, oxygen consumption rate; ROS, reactive oxygen species; SDHD, succinate dehydrogrenase complex subunit D; TBTA, Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine; TCEP, Tris(2-carboxyethyl) phosphine.

    Journal: International Journal of Molecular Medicine

    Article Title: Bufalin exerts antitumor effects in neuroblastoma via the induction of reactive oxygen species-mediated apoptosis by targeting the electron transport chain

    doi: 10.3892/ijmm.2020.4745

    Figure Lengend Snippet: Chemical proteomics highlights the ETC as a potential target. (A) Workflow diagram of nanoLC-MS/MS. (B) Venn diagram of proteins identified in three experimental repeats. (C) Gene Ontology analysis annotated cellular components enriched by CS-P1. (D) Diagram of ETC and the possible targets of CS-P1. (E) Seahorse assay of oxygen consumption and (F) OCR decrease after administration of bufalin in SK-N-BE cells. Rotenone was used as a positive control. (G) Intracellular ROS detection and (H) increase in ROS levels after CS-P1 treatment (329 nM, 72 h; magnification, x200). (I) Mitochondrial membrane potential detection with JC-1 probes and (J) reduction after CS-P1 treatment (329 nM, 72 h; magnification, x200). Data are presented as the mean ± SD (n=3). *** P<0.001 vs. DMSO. ATP5J, ATP synthase-coupling factor 6; COX7C, cytochrome c oxidase subunit 7C; DMSO, dimethyl sulfoxide; ETC, electron transport chain; LC-MS/MS, liquid chromatography-tandem mass spectrometry; OCR, oxygen consumption rate; ROS, reactive oxygen species; SDHD, succinate dehydrogrenase complex subunit D; TBTA, Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine; TCEP, Tris(2-carboxyethyl) phosphine.

    Article Snippet: Bufalin (MedChemExpress), N-acetyl-L-cysteine (NAC; cat. no. S0077; Beyotime Institute of Biotechnology) and the bufalin-derived probe CS-P1 were dissolved in dimethyl sulfoxide (DMSO) at a stock concentration of 100 mM and diluted with medium before experimentation.

    Techniques: Tandem Mass Spectroscopy, Positive Control, Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry